Cytogenetic study of Nocardia corallina.

نویسندگان

R B WEBB

J B CLARK

چکیده

Cytogenetics has proved to be a valuable aid in the study of higher organisms but has received only limited attention in microbiology. Witkin (1951), Ryan et al. (1954) and Ryan and Wainwright (1954) have successfully applied cytogenetic approaches to the study of bacteria. Haploid and diploid cultures of Escherichia coli strain K-12 were studied cytologically by Lederberg et al. (1951) in an effort to establish genetic ploidy on a cytological basis. The comparisons revealed consistent and unequivocal differences in the nuclear appearance of these two forms but failed to explain the ploidy of the cultures on a cytological basis. Clark and Webb (1955a) studied the large cells of Micrococcus aureus cytologically and radiobiologically, and presented evidence for the diploid and triploid nature of these forms. A cytogenetic study has been successfully applied to yeast by Lindegren (1949). The ploidy, life cycle, and sexual process have been substantiated by this approach although there is still some controversy in the field of yeast cvtology. The success with which the limited application of cytogeneties has met in microbiology indicates that certain of the basic problems of microbial genetics may be profitably approached by using the available tools of this field. Webb (1956) has completely reviewed the literature on cytogenetic approaches involving microorganisms. Preliminary cytological studies on Nocardia

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